ARNTL Knockout cell line (HCT116)

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ARNTL Knockout cell line (HCT116) Order

ARNTL Knockout cell line (HCT116)
Catalog#
YKO-KP267
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ARNTL Knockout cell line (HCT116)
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Detailed Product Information

Product category
KO Cell Lines
cell name
ARNTL Knockout cell line (HCT116)
Species
Human
Gene Name
ARNTL
Gene ID
406
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ARNTL gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ARNTL gene knockout ARNTL Knockout cell line (HCT116) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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